Movie S1.

Hemodynamic correction of Thy1-GCaMP6f fluorescence. (Left) Uncorrected GCaMP ∆F/F and (Center) hemodynamic-corrected GCaMP ∆F/F in an awake animal at resting state, accompanied by concurrent ∆[HbT] (Right) measured from optical hemodynamic imaging. Both the neural and hemodynamic sequences were temporally bandpass filtered from 0.02 to 2 Hz. Field of view shows entire, bilaterally exposed superficial cortex (thinned skull). The cross talk from changing hemodynamics can be seen as vascular structures in uncorrected raw GCaMP data but are removed after correction.