Movie S5.

Neurovascular coupling at low frequencies (shown with a 1.14-s delay to temporally align neural activity with later hemodynamics). Hemodynamics-corrected Thy1-GCaMP6f ΔF/F movie shown unfiltered (Left) and temporally low-pass filtered at 0.4 Hz (Middle). Simultaneously acquired Δ[HbT] is shown low-pass filtered at 0.4 Hz, but also temporally shifted 1.14 s (same trial as Fig. 3B and Movies S2–S4). This movie demonstrates strong spatiotemporal neurovascular coupling at low frequencies. It also validates that coupling is not an artifact of hemodynamic contamination of fluorescence, because correlations are not apparent without a temporal delay, and no vascular structures are seen in the patterns of neural activity.