Gamma-variate fitting of resting-state ∆[HbT] in urethane-anesthetized Thy1-GCaMP3 mouse brain after removal of slow hemodynamic trends. In the zoomed-in unilateral field of view, from Left to Right, the movie shows hemodynamics-corrected GCaMP fluorescence, measured Δ[HbT], the gamma-variate fit, and the residual difference between the [HbT] measurement and fit. All GCaMP fluorescence and Δ[HbT] signals were bandpass filtered at 0.04–2 Hz to remove slow trends. Movie clip corresponds to 0–60 s of the time courses in Fig. 5C.