Image sequence of <0.04-Hz slow trends in urethane-anesthetized Thy1-GCaMP3 mouse brain. From Left to Right: Δ[HbT] (temporally bandpass filtered, 0–2 Hz), the hemodynamic slow trend ([HbT] bandpass filtered, 0–0.04 Hz), hemodynamic changes with <0.04-Hz frequency removed ([HbT] bandpass filtered, 0.04–2 Hz), and gamma-variate fitting from Thy1-GCaMP3 recordings during the same epoch. Movie clip corresponds to 40–100 s of the time courses in Fig. 5 B–E.