Movie S5.

Plasma membrane insertions of tdt-GluA2 and pH-γ2S have different distributions. tdt-GluA2 and pH-γ2S were coexpressed in hippocampal neurons. Two days after transfection, plasma membrane insertions of tdt-GluA2 and pH-γ2S were simultaneously imaged under dual TIRFM. Left channel represents insertions of pH-γ2S, which excited by a laser at 488 nm. Right channel represents insertions of tdt-GluA2, which excited by a laser at 568 nm. The movie was taken at 500-ms exposure and 1.4-Hz acquisition frequency.