Movie S6.

Cotrafficking of EGFP-γ2S/tdt-γ2S and EGFP-GluA2/tdt-GluA2 after exiting the Golgi apparatus. Trafficking vesicles were imaged at 32 °C at 0.7-s interval after a preincubation at 20 °C. Top panel: cotrafficking of EGFP-γ2S and tdt-γ2S. Left channel represents a trafficking of EGFP-γ2S-containing vesicle. Middle channel represents the trafficking of the same vesicle containing tdt-γ2S. Right channel shows an overlaid movie of left and middle channels, representing a cotrafficking vesicle containing both EGFP-γ2S (green) and tdt-γ2S (magenta). Arrows point out the position of EGFP-γ2S/tdt-γ2S vesicle in each channel. Bottom panel: cotrafficking of EGFP-GluA2 and tdt-GluA2. Left channel represents the trafficking of an EGFP-GluA2-containg vesicle. Middle channel represents the trafficking of the same vesicle containing tdt-GluA2. Right channel shows an overlaid movie of left and middle channels, representing a cotrafficking vesicle containing both EGFP-GluA2 (green) and tdt-GluA2 (magenta). Arrows point out the position of EGFP-GluA2/tdt-GluA2 vesicle in each channel. (Scale bar: 2.5 µm.)