Movie S7.

Trafficking of EGFP-GluA2 and tdt-γ2S after exiting the Golgi apparatus. Trafficking vesicles containing EGFP-GluA2 or tdt-γ2S were simultaneously imaged at 32 °C at 0.7-s interval after a preincubation at 20 °C. Left channel represents the EGFP-GluA2 channel. Middle channel represents the tdt-γ2S channel. Right channel shows an overlaid movie of left and middle channels. EGFP-GluA2 is shown in green and tdt-γ2S is shown in magenta. Top panel: an example of a trafficking vesicle containing only tdt-γ2S, but not EGFP-GluA2. Arrows point out the position of tdt-γ2S-vesicle in each channel. Bottom panel: an example of a trafficking vesicle containing only EGFP-GluA2, but not tdt-γ2S. Arrows point out the position of EGFP-GluA2-vesicle in each channel. (Scale bar: 2.5 µm.)