Movie S1.

FUCCI cell cycle reporter expression in confluent NIH 3T3 cells. Confluent FUCCI mCherry::Cdt1(G1 phase)/amCyan::Geminin(S/G2 phase) reporter NIH 3T3 cell monolayers were synchronized and imaged between CT0 and 66 h. Cells expressing the G2 phase marker amCyan::Geminin (blue) account for <20% of the total number and no circadian rhythm in mCherry::Cdt1/ amCyan::Geminin ratio is observed (Fig. S3).