Movie S1.

Simulation of real-time acetylation: lattice-entry model. This simulation demonstrates (i) the dynamics of simulated αTAT1-GFP with a red stabilized microtubule (Top; microtubule, red; αTAT1-GFP, green), (ii) the associated pattern of acetylation as viewed by a simulated antiacetylated tubulin antibody (Middle; microtubule, red; antiacetyl antibody, blue), and (iii) a schematic output of the process (Bottom), in which microtubule protofilaments are shown in red; αTAT1 on the external surface of the microtubule is shown in green; and αTAT1, which has entered into the microtubule lumen, is shown in blue. Simulation length is 20 min real time.