Movie S1.

Intravascular retention and migration of GFP+ monocytes in uninflamed glomerular capillaries of a Cx3cr1GFP/+ mouse versus a Cx3cr1GFP/GFP mouse. Movies of a glomerulus in a kidney of a Cx3cr1GFP/+ (Left) and Cx3cr1GFP/GFP mouse (Right) showing GFP+ monocytes (green) undergoing retention within glomerular capillaries (blue, labeled with Qtracker-655). In the Cx3cr1GFP/+ mouse, several monocytes can be observed undergoing prolonged retention and migration in the glomerulus. In contrast, in the Cx3cr1GFP/GFP mouse, fewer monocytes are observed, and the single monocyte retained in the glomerulus has a greatly reduced dwell time relative to those in the Cx3cr1GFP/+ mouse. (Multiphoton microscopy; duration in real time, 27 min.) (Scale bars: 10 μm.)