Movie S2.

Neutrophil recruitment and oxidant generation in an inflamed control liposome-treated mouse. Movies show a glomerulus in a kidney of a WT mouse after control liposome treatment 1–2 h after receiving anti-GBM Ab. Several neutrophils (green, labeled with anti–Gr-1-A488) can be seen undergoing sustained retention and migration within glomerular capillaries (blue, labeled with Qtracker-655). Some neutrophils also display reactive oxygen species generation (red, visualized by DHE-associated fluorescence). (Left) A merged display of all channels (Gr-1, Qtracker, DHE). (Right) The same sequence in the absence of Gr-1 staining to allow easier visualization of the DHE signal. Arrows indicate DHE+ neutrophils. (Multiphoton microscopy; duration in real time, 19 min, 30 s.) (Scale bars: 10 μm.)