Movie S6.

Comparison of intravascular monocyte–neutrophil interactions in uninflamed and inflamed glomeruli. Movies show glomeruli in kidneys of a Cx3cr1GFP/+ mouse 1–2 h after receiving NSG as control (Left) or anti-GBM Ab (Right). In the NSG-treated mouse, two interactions between a GFP+ monocyte (green) and neutrophils (red, labeled with anti–Gr-1-PE) of ∼2 min duration (denoted by arrows) are detected. In the anti-GBM Ab–treated mouse, a monocyte is seen undergoing a prolonged interaction (denoted by an arrow) with a neutrophil. (Spinning disk confocal microscopy; duration in real time, 21 min, 30 s). (Scale bars: 10 μm.)