Movie S7.

Neutrophil oxidant generation after interaction with a monocyte. Movies show a glomerulus in a kidney of a Cx3cr1GFP/+ mouse 1–2 h after receiving anti-GBM Ab. A neutrophil (blue, labeled with anti–Gr-1-Dylight 650) (indicated by an arrow) can be seen undergoing interaction with a GFP+ monocyte (green) and rapidly becoming positive for DHE-associated fluorescence, indicative of reactive oxygen species generation. (Left) A merged display of all channels (Gr-1, Qtracker, DHE). (Right) The same sequence in the absence of Gr-1 staining to allow easier visualization of the DHE signal. (Spinning disk confocal microscopy; duration in real time, 19 min, 30 s). (Scale bars: 10 μm.)