Movie S2.

Blocking CCN1 impairs the recruitment of monocytes and neutrophils in response to R848 stimulation. Confocal intravital microscopy of mesenteric vein. The movie starts 150 min after topical stimulation of the monitored vessel with R848 (100 μg). Control sheep Ig (Left) or antibodies blocking CCN1 (50 μg/mL) (Right) were i.v. injected simultaneously with R848 stimulation. Ly6Clow monocytes are shown by GFP (green). Transferred neutrophils are shown by CellTracker Orange (red). Movie duration is 30 min. (Scale bar: 50 μm.) (See Fig. 2.)

CCN1/CYR61-mediated meticulous patrolling by Ly6Clow monocytes fuels vascular inflammation

Beat A. Imhof, Stephane Jemelin, Romain Ballet, Christian Vesin, Marco Schapira, Melis Karaca, and Yalin Emre

PNAS. 2016. DOI: 10.1073/pnas.1607710113