Movie S3.

Z stacks of confocal sections (each of depth = 0.8 μm) of wild-type w1118 wing discs depicting 5-min pulsed 10-min chased endosomes (Fig. 1A, step 4) of Dex (blue), Wg (red), and DFz2 (green) from apical planes to basal planes. (Scale bar, 50 μm.) Movie is played at 10 frames per second.

Endocytosis of Wingless via a dynamin-independent pathway is necessary for signaling in Drosophila wing discs

Anupama Hemalatha, Chaitra Prabhakara, and Satyajit Mayor

PNAS. 2016. 113:E6993-E7002 DOI: 10.1073/pnas.1610565113