Z stacks of confocal sections (each of depth = 0.8 μm) of wild-type w1118 wing discs depicting 5-min pulsed 10-min chased endosomes (Fig. 1A, step 4) of Dex (blue), Wg (red), and DFz2 (green) from apical planes to basal planes. (Scale bar, 50 μm.) Movie is played at 10 frames per second.