Movie S4.

ForG promotes actin filament elongation in the presence of PFN (refers to Fig. 5C). Time-lapse recordings of single actin filaments by in vitro TIRFM. The elongation of actin filaments (1.3 μM actin, 23% Atto488-labeled) and 5 μM PFN I either in the absence or presence of 10 nM ForG-1P or 10 nM ForG-3P was recorded over a period of 10 min. Time is minutes and seconds. (Scale bar: 10 μm.)