Movie S3.

Addition of cholesterol to the DOPC SLBs reduced the diffusivity of lipids. Time-lapse movie showing FRAP for two different lipid compositions: DOPC:Cholesterol:DSPE-PEG-2000-Biotin at 79:20:1 (20% CH, Left, S3.1) and DOPC:Cholesterol:DSPE-PEG-2000-Biotin at 39:60:1 (60% CH, Right, S3.2). Fluorescence recovery of the photobleached region is faster in the SLB with 20% CH compared with 60% CH. (Scale bar, 10 µm.)