Movie S2.

A video showing cryo-FIB-SEM serial sections that were aligned to form the same data cube of X = 20 µm, Y = 12 µm, Z = 11 µm, with a voxel size of 10 × 10 × 20 nm as in Movie S1. Segmentation of the reconstructed 3D is shown for several vesicles and a part of the plasma membrane. The purple color shows the outline of the plasma membrane of a PMC. In the continuation of the video, the purple color cumulatively designates the plasma membrane in 3D and this then reveals a complex of connected vesicles (light purple) on the inside of the cell that has openings through the plasma membrane.

Calcium transport into the cells of the sea urchin larva in relation to spicule formation

Netta Vidavsky, Sefi Addadi, Andreas Schertel, David Ben-Ezra, Muki Shpigel, Lia Addadi, and Steve Weiner

PNAS. 2016. DOI: 10.1073/pnas.1612017113