Movie S2.

Corresponding fluorescence video of the same cells. When the PVM becomes leaky, there is a sudden drop in intensity. At 5 min, 10 s, the cell at the top left (indicated with arrow) shows release of fluorescence from the vacuole before rounding up and final parasite escape. At 5 min, 15 s, another cell to the right of the center (indicated by arrow) displays release of fluorescence into the blood cell. Although egress does not occur during the recording period, the cell rounds up, indicating likely egress shortly after the end of the recording. The overall fluorescence intensity fades gradually over time because of photobleaching.