Movie S1.

Schematic depiction of a single trajectory produced by the stochastic model of T4 replication focusing on the dynamics of Okazaki fragment initiation. Total simulation time was 200 s, corresponding to the helicase unwinding 30 Kbp of double-stranded DNA. Viewed over ∼1,600 bp downstream from the helicase, this video illustrates the stochastic nature of priming events, clamp–clamp loader capturing these primers, and polymerase recycling to the primer–clamp–clamp loader complexes to initiate Okazaki fragment synthesis. Note that the specific details of Okazaki fragment initiation within this single trajectory do not necessarily correspond to the ensemble average values presented in the text, which were determined using 10,000 trajectories.