Movie S2.

Schematic depiction of a single trajectory produced by the stochastic model of T4 replication focusing on the dynamics of holoenyme recycling. Total simulation time was 1,000 s, corresponding to the helicase unwinding 150 Kbp of double-stranded DNA. Viewed over ∼16 Kbp downstream from the helicase, this video illustrates the stochastic nature of priming events and holoenzyme recycling, resulting in a diverse population of Okazaki fragment lengths as well as a mixture of both collision and signaling events, with the latter process creating gaps between successive fragments. Note that the specific fragment lengths within this single trajectory do not necessarily correspond to the ensemble average values presented in the text, which were determined using 10,000 trajectories.