Movie S2.

Trajectory of Tpm movement on the surface of F-actin (Movie S1) is superimposed on the atomic model of rigor-myosin–F-actin complex (7). Sterical clashes between myosin head and Tpm in the c-blocked (blue) and the c-closed (green) structural states are marked with big yellow arrows, whereas a small sterical hindrance between loop-4 of myosin and Tpm in the c-open structural state is indicated with a small yellow arrow.