Movie S2.

Representative example movie of a EVL/VASP dKO T-cell attempting diapedesis in vitro, as visualized by time-lapse phase and fluorescent confocal microscopy. Fluorescence and phase shown on left, phase alone shown on right. Fluorescently-labeled activated WT and EVL/VASP dKO T cells were flowed on brain microvascular endothelial monolayers and imaged by time-lapse fluorescent and phase contrast microscopy. WT T cells are in green and dKO T cells are in red. Red arrows indicate diapedesis attempts (extension of protrusions). (Scale bar, 10 μm.) Timestamps are minutes:seconds.