Movie S1.

Side-view imaging of A7 cells grown on a collagen-coated glass substrate under osmotic compression with the addition of 10% PEG 300. We image two A7 cells from the side for ∼20 min under isotonic condition, then add 10% PEG 300 to compress them, and keep imaging them for ∼2 h in the medium with PEG. The movie shows that cells are compressed suddenly (within 20 s) after the addition of PEG, and the compressed state is stably maintained for the rest of imaging time.

Cell volume change through water efflux impacts cell stiffness and stem cell fate

Ming Guo, Adrian F. Pegoraro, Angelo Mao, Enhua H. Zhou, Praveen R. Arany, Yulong Han, Dylan T. Burnette, Mikkel H. Jensen, Karen E. Kasza, Jeffrey R. Moore, Frederick C. Mackintosh, Jeffrey J. Fredberg, David J. Mooney, Jennifer Lippincott-Schwartz, and David A. Weitz

PNAS. 2017. 114:E8618-E8627 DOI: 10.1073/pnas.1705179114