Movie S1.

Time-lapse image of a centered, dual-labeled HIV particle challenging a cell. Plotting of the measured mean intensities with background subtracted (y axis) within the voxels of the tracked individual particle is shown. (Top Left) Two drops of GFP signal are discerned in the presence of Vpr. (Right) Particle box shows the signal of GFP (green) and Vpr (red) over time. (Bottom Left) Smaller boxes show the signal (gray) of each individual fluorescence measurement as labeled.