Movie S3.

Time-lapse image of a centered, dual-labeled (iGFP and IN-mRuby) HIV particle challenging a cell with NH4Cl-synchronized fusion, where fusion occurred after the first frame. Plotting of measured mean intensities with background subtracted (y axis) of the voxels of a tracked individual particle is shown for different time points in minutes (x axis). (Left) Two drops of GFP signal are discerned in the presence IN label. (Top Right) Particle box shows the signal of GFP (green) and IN (red) over time. (Bottom Right) Smaller boxes show the signal (gray) of each individual fluorescence measurement as labeled.