Movie S5.

Time-lapse image of a centered, dual-labeled HIV particle challenging a cell with NH4Cl-synchronized fusion in the presence of NVP, followed by NVP removal at 50 min. (Left) Plotting of the measured mean intensities with background subtracted (y axis) within the voxel of the tracked individual particle. A delay of the integrity loss of viral capsid was observed in the presence of Vpr. (Top Right) Particle box shows the signal of GFP (green) and Vpr (red) over time. (Bottom Right) Smaller boxes show the signal in gray of each individual fluorescence measurement as labeled.