Movie S7.

Time-lapse image of a centered, dual-labeled HIV (WTEnv) particle during infection of a primary activated CD4+ T cell shows the timing of loss of capsid integrity. (Top Left) Plotting of measured mean intensities with background subtracted (y axis) of the voxels of the tracked individual particle is shown at different time points in minutes (x axis); two drops of GFP signal are discerned in the presence of Vpr. (Bottom Left) Particle boxes show the signal of GFP (green) and Vpr (red) over time. (Bottom Left and Right) Other boxes show the signal (gray) of each individual fluorescence measurement as labeled. During time-lapse imaging, a brightfield reference image was taken for every time point (gray) and mixed with z-projected fluorescence acquisitions.