Movie S9.

Time-lapse image of a centered, triple-labeled HIV particle shows simultaneous loss of capsid integrity (GFP) and CA-ReAsH before fixation and p24CA staining. (Left) Plotting of measured mean intensities with background subtracted (y axis) of the voxels of the tracked individual particle is shown at different time points in minutes (x axis). (Top Right) Particle box shows the signal of GFP (green), Vpr (red), and CA-ReAsH (blue) over time. (Bottom Right) Smaller boxes show the signal (gray) of each individual fluorescence measurement as labeled.