hCO contracting within the Heart-Dyno culture insert. Contractile properties can be approximated by tracking the movement of the PDMS elastomeric poles. The microscope is focused on the top of the elastomeric poles. The video was taken over a period of 10 s and is displayed in real time (50 frames per 1 s).
Richard J. Mills, Drew M. Titmarsh, Xaver Koenig, Benjamin L. Parker, James G. Ryall, Gregory A. Quaife-Ryan, Holly K. Voges, Mark P. Hodson, Charles Ferguson, Lauren Drowley, Alleyn T. Plowright, Elise J. Needham, Qing-Dong Wang, Paul Gregorevic, Mei Xin, Walter G. Thomas, Robert G. Parton, Lars K. Nielsen, Bradley S. Launikonis, David E. James, David A. Elliott, Enzo R. Porrello, and James E. Hudson
PNAS. 2017. DOI: 10.1073/pnas.1707316114