Movie S8.

Advanced insulitis II. Representative time-lapse imaging of T cell, DCs, and MF dynamics during advanced insulitis in a 12-wk-old CD4-tdTomato-Foxp3-iGFP-Mertk-GFP-Flt3-BFP2 mouse. In this particular lesion, which exhibits similarities akin to peri-insulitis defined by traditional H&E analysis (extensive cellular infiltrate, typical round structure, and appearance of cells completely confined to beyond the islet perimeter), there is a dense, actively mobile population of T cells that comprise the core insulitic lesion, which surrounds the islet. In addition, there is intermittent trafficking of T cells to and from the lesion which occurs through the exocrine compartment of pancreas. The second half of the movie depicts the same imaging series with signal from the CD4-tdTomato removed. Note the active migration behavior of the DCs, whereas the MFs are almost entirely sessile (migrating green cells were manually inspected and confirmed to be Tregs). The movie corresponds with data shown in Fig. 4C.