Movie S5.

Time-lapse video showing RICM (platelet adhesion, Left), 561 nm TIRF (4.7 pN tension signal, Middle), and 488 nm TIRF (Furo-3 calcium signal, Right) for a human platelet during the initial stages of activation. The duration of the video was 13 min, and the data were acquired at six frames per minute. (Scale bar: 5 μm.)