Movie S11.

Movie for Fig. 5B, showing preparation of DEVs and reactivation of their motility with ATP under Ca2+-free conditions. The movie is run at ×2 speed. Experimental steps are shown in the captions. In brief, spheroids are transferred into the Igepal solution for demembranation at 12 s and then transferred into the Ca2+-free buffer at 37 s. ATP is added at 58 s. Magnified images are shown from 2 min 2 s.