Movie S12.

Movie for Fig. 5B, showing preparation of DEVs and reactivation of their motility with ATP in the presence of 10−6 M Ca2+. The movie is run at ×2 speed. Experimental steps are shown in the captions. In brief, spheroids are transferred into the Igepal solution for demembranation at 8 s and then transferred into the buffer containing 10−6 M Ca2+ at 19 s. ATP is added at 30 s. Magnified images are shown from 1 min 26 s.