Kanamycin-resistant E. coli Top10 cells expressing ilux in the presence of kanamycin. E. coli Top10 cells expressing ilux from pQE(−) containing a kanamycin instead of ampicillin resistance marker were imaged under an LB agar pad containing 100 µg/mL kanamycin. Single images were taken with 10-min exposure time. (Scale bar: 2 µm.)