Movie S1.

HeLa cell expressing GFP-tubulin and mCh-Eg5 WT imaged every 60 s after monastrol washout played back at eight frames per second (480× real time).

A posttranslational modification of the mitotic kinesin Eg5 that enhances its mechanochemical coupling and alters its mitotic function

Joseph M. Muretta, Babu J. N. Reddy, Guido Scarabelli, Alex F. Thompson, Shashank Jariwala, Jennifer Major, Monica Venere, Jeremy N. Rich, Belinda Willard, David D. Thomas, Jason Stumpff, Barry J. Grant, Steven P. Gross, and Steven S. Rosenfeld

PNAS. 2018. 115:E1779-E1788 DOI: 10.1073/pnas.1718290115