Movie S1.

Time-lapse imaging of neutrophils incubated with EFIG-AM-treated brain capillary endothelial cells. Lysosomes of cocultured hCMEC/D3-MDR1-EGFP and hCMEC/D3 WT cells were labeled by LysoTracker (blue) and cells were subsequently treated with EFIG-AM (EFIG; red) after excess LysoTracker was removed by washing. The cocultures were then incubated with freshly isolated blood-derived human neutrophils that were labeled by eFluor670 (white) before. The triple-culture was examined with a confocal microscope and time-lapse imaging at 37°C. The initially white eFluor670 labeled neutrophils show over time a punctate colocalizing staining for Pgp and EFIG as well as for LysoTracker. The neutrophil highlighted by arrow 1 is shown as detailed view in Fig. 7. Neutrophil extend pseudopods towards surface of hCMEC/D3 cell likely scanning for target antigens (arrow 2).