Movie S1.
Raw images annotated with the tracking results for three families (red, blue, and magenta outlines) for WT in FC1 (T = 29.5 h). Each outline is a single bacterium boundary, and other bacteria are gray. The movie is cropped to the families’ spatial positions. The movie’s upper-left corner corresponds to (x, y) = (361, 7) (pixels) in the raw images (origin in the upper-left corner). The lower-right corner shows the time stamp (hour:minutes:seconds) and frame number (4,004–7,600, every four frames, 3 s per frame), where frame 1 corresponds to t = 20.5 h. The upper-left corner shows a 5-μm scale bar (movie dimensions of 664 × 608 pixels). The movie is playing back at 20 frames per s (1 min of real-time recording per second of playback). Shown here is a snapshot of frame 5,804 (25 h:20 min:12 s).
Multigenerational memory and adaptive adhesion in early bacterial biofilm communities
PNAS. 2018. 115:4471-4476 DOI: 10.1073/pnas.1720071115