Movie S2.

Platelet aggregates readily form in the brain microvasculature of FcγRIIATGN mice. Intravital microscopy in brain microvasculature of FcγRIIATGN/CD41-YFP mice injected with ICs. The response was monitored for at least 30 min after IC injection. Multiple regions of interest were observed for each mouse, and the result is representative of at least three independent experiments. Thrombi were observed 5 min after IC injection in FcγRIIATGN/CD41-YFP mice. YFP expression is shown in green, and Evans Blue expression is shown in red. (Scale bar: 25 μm.)

Platelets release pathogenic serotonin and return to circulation after immune complex-mediated sequestration

Nathalie Cloutier, Isabelle Allaeys, Genevieve Marcoux, Kellie R. Machlus, Benoit Mailhot, Anne Zufferey, Tania Levesque, Yann Becker, Nicolas Tessandier, Imene Melki, Huiying Zhi, Guy Poirier, Matthew T. Rondina, Joseph E. Italiano, Louis Flamand, Steven E. McKenzie, Francine Cote, Bernhard Nieswandt, Waliul I. Khan, Matthew J. Flick, Peter J. Newman, Steve Lacroix, Paul R. Fortin, and Eric Boilard

PNAS. 2018. 115:E1550-E1559 DOI: 10.1073/pnas.1720553115