Resident CD8 T cells form long-lasting contacts with macrophages in the stroma of human lung tumors. The slices stained with anti-CD8 (red), anti-CD206 (green), and anti-EpCAM (blue) antibodies were imaged with a spinning disk confocal microscope. Tracks are color-coded according to CD8 T cell displacement length. Frame interval is 30 s. The animations represent 3D reconstructions of sequential z series. A still image is shown in Fig. 2C. Note the presence of autofluorescent structures that appear in magenta around the tumor islet.
Elisa Peranzoni, Jean Lemoine, Lene Vimeux, Vincent Feuillet, Sarah Barrin, Chahrazade Kantari-Mimoun, Nadège Bercovici, Marion Guérin, Jérôme Biton, Hanane Ouakrim, Fabienne Régnier, Audrey Lupo, Marco Alifano, Diane Damotte, and Emmanuel Donnadieu
PNAS. 2018. 115:E4041-E4050 DOI: 10.1073/pnas.1720948115