Movie S3.

CP-depleted HeLa cells fail cytokines after furrowing. Epifluorescence movies of HeLa cells stably expressing GFP-tubulin (green) and H2B-mCherry (red) transfected with nontargeting control siRNA (Upper) or CP siRNA (Lower) imaged after 50 h RNAi. The major cytokinesis failure phenotype is after furrowing (Lower; representative of 57 of 101 captured live cells). Frames were acquired every 3 min, and playback speed is 10 frames per second. Movies were acquired from three independent experiments. (Scale bar: 10 μm.)