Movie S4.

CIT-K–depleted cells fail cytokinesis after furrowing without extended abscission delay, and IST1-depleted cells fail after an extended abscission delay. HeLa cells stably expressing GFP-tubulin (green) and H2B-mCherry (red) transfected with CIT-K siRNA (Upper) were imaged after 24 h RNAi (representative of 32 of 32 captured live cells), and HeLa transfected with IST1 siRNA (Lower) were imaged after 50 h RNAi (representative of 30 of 30 captured live cells). Frames were acquired every 3 min, and playback speed is 10 frames per second. Movies were acquired from three independent experiments. (Scale bar: 10 μm.)