3D SIM projection of CHMP2A localization to midbody in control siRNA-treated cells. Movie of a 3D projection produced from 3D SIM-reconstructed 4-μm z series from HeLa cell transfected with control siRNA. Tubulin (gray) and CHMP2A (red) are shown. The image represents the two-ring stage of CHMP2A localization, and it is representative of 10 images.