Movie S1.

Tw2-tdTO+ cells differentiated into CMs when maintained in CM induction media, as identified by expression of the CM marker cTnT (red). Movie shows that some of the differentiated CMs began beating after 2 weeks of culture.

Identification of a multipotent Twist2-expressing cell population in the adult heart

Yi-Li Min, Priscilla Jaichander, Efrain Sanchez-Ortiz, Svetlana Bezprozvannaya, Venkat S. Malladi, Miao Cui, Zhaoning Wang, Rhonda Bassel-Duby, Eric N. Olson, and Ning Liu

PNAS. 2018. 115:E8430-E8439 DOI: 10.1073/pnas.1800526115