Movie S2.

Maximum intensity projected 3D visualization of eYFP-expressing Arc POMC neurons and their connectivity in 4mm-thick CLARITY processed brain tissue from a viral POMC-ChR2 mouse. This representative volume-rendered movie shows prominent ipsilateral arcuate connectivity with the paraventricular hypothalamus, zona incerta, periaqueductal grey and lateral septum in the viral POMC-ChR2 mouse brain.