Movie S5.

The time-laps images of the binding of cofilin-EGFP to mDia1(-), untrapped, buckled and stuck F-actin (Fig. 2A). Cofilin-EGFP (green) and DyLight 550-labeled actin (red) are shown. When 2 μM cofilin (10% cofilin-EGFP) is added to the flow cell, the green background increases on the movie. White lines show the shape of each F-actin in the first frame. Arrowheads show the barbed end of each F-actin. Scale bar is 5 μm.