Movie S6.
Single-molecule speckle (SiMS) observation of EGFP-mDia1Full, EGFP-mDia1ΔC63 and EGFP-mDia1ΔN3 in live XTC cells. For SiMS imaging, the expression level of EGFP-mDia1 mutants was reduced by the defective CMV promoter. Time is in seconds. Scale bar, 10 μm.
Helical rotation of the diaphanous-related formin mDia1 generates actin filaments resistant to cofilin
PNAS. 2018. 115:E5000-E5007 DOI: 10.1073/pnas.1803415115