To view this video please enable JavaScript, and consider upgrading to a web browser that supports HTML5 video Movie S7. Single-molecule speckle (SiMS) observation of DyLight550-actin introduced into non-transfected XTC cells (A) and XTC cells overexpressed mDia1 Full (B), mDia1ΔC63 (C) and mDia1ΔN3 (D), respectively. Scale bar, 20 μm. Helical rotation of the diaphanous-related formin mDia1 generates actin filaments resistant to cofilin Hiroaki Mizuno, Kotaro Tanaka, Sawako Yamashiro, Akihiro Narita, and Naoki Watanabe PNAS. 2018. 115:E5000-E5007 DOI: 10.1073/pnas.1803415115 Download Original Video Browse All Videos »