Movie S1.

Expected collective movements of cohesin hinge at the south interface, as deduced by normal mode analysis using ANM2.1. Atomic models of the wild-type mouse cohesin hinge appear yellow (SMC1) and green (SMC3). The model of the S568E mutant, which is equivalent to Psm3-A561E, one of the Cut1 suppressors, appears blue to red depending on the amplitude of movement (red indicating large movement). Both atomic models were generated by all-atom molecular dynamics simulations for 100 ns starting from the crystal structure of the wild type (PDB code: 2WD5). Purple broken lines show distances between residues on helix I of SMC1 (V622 and F630) and those on helix E (M573 and D565) of SMC3 in the mutant. This movie shows that the mutation causes much larger movements of helices A and E and those of coiled-coils connecting the hinge and the head domains of SMC3.