Live imaging of early endosome budding in primary porcine RPE cultures by spinning disk microscopy. Cells expressing RFP-Rab5 (red; CellLightTM BacMam 2.0 Early Endosome-RFP, ThermoFisher, Waltham, MA) were labeled with CellMask Deep Red to delineate the plasma membrane (pseudo-colored green) and the formation of Rab5-positive early endosomes was monitored for 5 min.
Gulpreet Kaur, Li Xuan Tan, Gurugirijha Rathnasamy, Nilsa La Cunza, Colin J. Germer, Kimberly A. Toops, Marie Fernandes, Timothy A. Blenkinsop, and Aparna Lakkaraju
PNAS. 2018. 115:9014-9019 DOI: 10.1073/pnas.1805039115